Examining Malignant Mesothelioma for Gene Mutations

Examining Malignant Mesothelioma for Gene Mutations

An interesting study is called, “Diagnosis of diffuse malignant mesothelioma: experience of a US/Canadian Mesothelioma Panel.” By McCaughey WT, Colby TV, Battifora H, Churg A, Corson JM, Greenberg SD, Grimes MM, Hammar S, Roggli VL, Unni KK. – Mod Pathol. 1991 May;4(3):342-53.  Here is an excerpt: “Abstract – The experience of the US/Canadian Mesothelioma Panel with its first 200 cases is reviewed. The light microscopic diagnosis, histochemical findings, immunohistochemical findings, and electron microscopic features of malignant mesotheliomas are reviewed in the context of differential diagnosis. Reasons for referral of case material to the panel and lessons from follow-up of difficult and controversial cases are reported. Recommendations to general pathologists are made regarding evaluation and review of possible mesotheliomas.”

An interesting study is called, “Molecular biology studies on mesothelioma tumor samples: preliminary data on H-ras, p21, and SV40.” By Cristaudo A, Vivaldi A, Sensales G, Guglielmi G, Ciancia E, Elisei R, Ottenga F – Institute of Endocrinology, University of Pisa, Italy. J Environ Pathol Toxicol Oncol. 1995;14(1):29-34.  Here is an excerpt: “Abstract – The ras gene is one of the oncogenes most commonly detected in human cancers; this protooncogene is converted to active oncogene by point mutations occurring at either codon 12, 13, or 61. SV40 is a DNA-transforming simian virus, a 105 bp sequence of which has been shown recently to be present in a significant fraction of human mesothelioma cells. Eleven human malignant mesotheliomas were examined for H-ras gene mutations at codon 12, 13, and 61 and for the presence of SV40-like sequences. DNA prepared from formalin-fixed and paraffin-embedded tissue was amplified by means of PCR and analyzed using designed restriction fragment length polymorphism. No mutation with respect to H-ras was found in any tumor sample, but the majority of mesothelioma cells contained SV40-like sequences.”

Another interesting study is called, “Value of the MOC-31 monoclonal antibody in differentiating epithelial pleural mesothelioma from lung adenocarcinoma.” By Ordóñez NG – University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.  Hum Pathol. 1998 Feb;29(2):166-9.  Here is an excerpt: “Abstract – MOC-31 is a monoclonal antibody that has recently become commercially available that recognizes an epithelial-associated, transmembrane glycoprotein often expressed in epithelial tumors. Although some authors have indicated that MOC-31 immunostaining can assist in distinguishing epithelial mesotheliomas from metastatic adenocarcinomas to the pleura, others have concluded that this marker has no value in separating these conditions. To determine whether MOC-31 immunostaining can assist in discriminating epithelial pleural mesothelioma from lung adenocarcinoma or from other carcinomas metastatic to the pleura, 38 epithelial pleural mesotheliomas, 40 pulmonary adenocarcinomas, 55 nonpulmonary adenocarcinomas, six squamous cell carcinomas of the lung (SCCLs), three small-cell lung carcinomas (SCLCs), 19 bronchial carcinoids (BCs), and 15 transitional cell carcinomas (TCCs) were studied. Reactivity was obtained in two (5%) of the mesotheliomas, in all 40 (100%) pulmonary adenocarcinomas, in 45 (82%) nonpulmonary adenocarcinomas, in six (100%) SCCLs, three (100%) SCLCs, 15 (83%) BCs, and 10 (67%) TCCs. The staining in the two positive mesotheliomas was restricted to a few cells, in contrast to the pulmonary adenocarcinomas and most of the other carcinomas where it was often strong and diffuse. It is concluded that MOC-31 can be useful in separating epithelial pleural mesothelioma from pulmonary adenocarcinoma or from other epithelial malignancies involving the pleura.”

We all owe a debt of gratitude to these fine researchers.  If you found any of these excerpts interesting, please read the studies in their entirety.

Monty Wrobleski is the author of this article.  For more information please click on the following links

Depuy Hip Recall Attorney

Depuy Hip Recall Lawyer

Depuy Hip Recall Lawyer

 


Article from articlesbase.com

Comments are closed.

Categories